| Number | Name | Manufacture/Origin | OrderNr. | Architecture (Tag, Cleavage) | Resistance | Promotor | Backbone | seq. Primer | Application | Host | Usage | Box | Position |
|---|
| VD0001 | pRSET His8
|
|
| n-term His8, EK (Enterokinase) | amp | T7 |
|
| high-level prokaryotic expression | E.coli |
| 1 | A1 |
| VD0002 | pCoofy1 | Sabine Suppmann, Addgene | 43974 | n-term His6 | kan |
| pETM14 |
|
| E.coli |
| 1 | A2 |
| VD0003 | pCoofy18 | Sabine Suppmann, Addgene | 43975 | n-term His10 | kan |
| pCoofy1 |
|
| E.coli |
| 1 | A3 |
| VD0004 | pCoofy31 | Sabine Suppmann, Addgene | 43993 | n-term OneStrep, c-term His10 | kan |
| pCoofy12 |
|
|
|
| 1 | A4 |
| VD0005 | pCoofy61 | Sabine Suppmann, Addgene | 122013 | n-term His6 | kan |
| pETM-NG |
|
|
|
| 1 | A5 |
| VD0006 | pFastBac1 | Joop van den Heuvel, HZI |
|
| amp in E.coli, Gent | Polyhedrin | pFastBac |
|
|
|
| 1 | A6 |
| VD0007 | pFastBacGST | Joop van den Heuvel, HZI |
| n-term GST | amp in E.coli, Gent | Polyhedrin | pFastBac |
|
|
|
| 1 | A7 |
| VD0008 | pcDNA3.1(+) | Joop van den Heuvel, HZI |
|
| amp in E.coli | CMV |
|
| Mammalian expression vector | Mam |
| 1 | A8 |
| VD0009 | pFlpBtm-II | Joop van den Heuvel, HZI |
|
|
|
|
|
|
|
|
| 1 | A9 |
| VD0010 | pET22b(+) | BNITM, Sigma Aldrich | 69744-3 | n-term pelB SS, c-term His6 | amp | T7 |
|
| pelB for potential periplasmic localization | E.coli |
| 1 | B1 |
| VD0011 | pET28c |
|
| n-term Thrombin, n-term His6, c-term His6 | kan | T7 |
|
|
| E.coli |
| 1 | B2 |
| VD0012 | pETM44 |
|
| n-term His6, n-term MBP, c-term ccdb, c-term His6 | kan | T7 |
|
|
| E.coli |
| 1 | B3 |
| VD0013 | pTT5BamAvr | Joop van den Heuvel, HZI |
|
| amp in E.coli | CMV | pTT5 |
|
| Mam |
| 1 | B4 |
| VD0014 | pTT5 | Joop van den Heuvel, HZI |
|
|
| CMV |
|
|
| Mam |
| 1 | B5 |
| VD0015 | pDAM02 |
|
|
|
|
|
|
|
|
|
| 1 | B6 |
| VD0016 | pCAD04 |
|
|
|
|
|
|
|
|
|
| 1 | B7 |
| VD0017 | pCAD09 |
|
|
|
|
|
|
|
|
|
| 1 | B8 |
| VD0018 | MoClo#1 |
|
| nStrep-TEV | amp |
|
|
|
|
|
| 1 | B9 |
| VD0019 | MoClo#2 |
|
| nStrep-MBP-TEV | amp |
|
|
|
|
|
| 1 | C1 |
| VD0020 | pFastBac HT-A | Gibco | 12562-029 | n-term His6 + TEV site | amp in E.coli | Polyhedrin |
|
|
| Insect |
| 1 | C2 |
| VD0021 | pFastBac HT-B | Gibco | 12562-029 | n-term His6 + TEV site | amp in E.coli | Polyhedrin |
|
|
| Insect |
| 1 | C3 |
| VD0022 | pFastBac HT-C | Gibco | 12562-029 | n-term His6 + TEV site | amp in E.coli | Polyhedrin |
|
|
| Insect |
| 1 | C4 |
| VD0023 | pFastBac HT-CAT | Gibco | 12562-029 | CAT gene (chloramphenicol acetyltransferase) | amp in E.coli |
|
|
| control pasmid | Insect |
| 1 | C5 |
| VD0024 | pFastBac-Dual | Gibco | 10712-024 | MCS - p10, Polyhedrin - MCS | amp in E.coli | Polyhedrin and p10 |
|
| double expression vector with two promotors | Insect |
| 1 | C6 |
| VD0025 | pFastBac-Dual Gus/CAT | Gibco | 10712-024 | chloramphenicol acetyltransferase and β-glucuronidase expression control | amp in E.coli | Polyhedrin and p10 |
|
| control plasmid | Insect |
| 1 | C7 |
| VD0026 | pEG BacMam | Eric Gouaux/OHSU |
|
| amp in E.coli | Polyhedrin, P10, CMV |
|
| Virus formation in Insect cells to infect Mammalian cells | Insect, Mam |
| 1 | C8 |
| VD0027 | pEG BacMam c-term 3c - eGFP - His8 | Eric Gouaux/OHSU |
| c-term 3c - eGFP - His8 | amp in E.coli | Polyhedrin, P10, CMV |
|
| Virus formation in Insect cells to infect Mammalian cells | Insect, Mam |
| 1 | C9 |
| VD0028 | pEG BacMam n-term His8 - eGFP - 3c | Eric Gouaux/OHSU |
| n-term His8 - eGFP - 3c | amp in E.coli | Polyhedrin, P10, CMV |
|
| Virus formation in Insect cells to infect Mammalian cells | Insect, Mam |
| 1 | D1 |
| VD0029 | pEZT-BM | Ryan Hibbs, Addgene | 74099 | Polyhedrin promoter replaced with CMV-containing cassette from pVLAD6 vector. Multiple cloning site replaced with custom. Synthetic, codon-optimized eGFP inserted after p10 promoter | amp in E.coli | P10, CMV |
|
| Multi-purpose rapid titer bacmam expression vector |
|
| 1 | D2 |