Page tree

You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 38 Next »

Please find below a list of all vectors available in the PPCF for members of CSSB groups. Please note that due to the existence of restricting material transfer agreements (MTAs) for some vectors, those vectors can only be provided for use in the Protein Production Core Facility. If you are interested in using these vectors or strains in a lab other than the PPCF, please get in contact so we can help you to put all needed MTAs in place. Some MTAs are coupled with fees. We are happy to advise you on this issue.
NumberNameManufactureOrderNr.Architecture (Tag, Cleavage)ResistancePromotorOriginBackboneseq. PrimerApplicationHostUsageBoxPosition
VD0001pRSET His8


n-term His8, EK (Enterokinase)ampT7


high-level prokaryotic expressionE.coli
1A1
VD0002pCoofy1Sabine Suppmann, Addgene43974n-term His6kan

pETM14

E.coli
1A2
VD0003pCoofy18Sabine Suppmann, Addgene43975n-term His10kan

pCoofy1

E.coli
1A3
VD0004pCoofy31Sabine Suppmann, Addgene43993n-term OneStrep, c-term His10kan

pCoofy12



1A4
VD0005pCoofy61Sabine Suppmann, Addgene122013n-term His6kan

pETM-NG



1A5
VD0006pFastBac1Joop van den Heuvel, HZI

amp in E.coli, GentPolyhedrin
pFastBac



1A6
VD0007pFastBacGSTJoop van den Heuvel, HZI
n-term GSTamp in E.coli, GentPolyhedrin
pFastBac



1A7
VD0008pcDNA3.1(+)Joop van den Heuvel, HZI

amp in E.coliCMV


Mammalian expression vectorMam
1A8
VD0009pFlpBtm-IIJoop van den Heuvel, HZI









1A9
VD0010pET22b(+)BNITM, Sigma Aldrich69744-3n-term pelB SS, c-term His6ampT7


pelB for potential periplasmic localizationE.coli
1B1
VD0011pET28c

n-term Thrombin, n-term His6, c-term His6kanT7



E.coli
1B2
VD0012pETM44

n-term His6, n-term MBP, c-term ccdb, c-term His6kanT7



E.coli
1B3
VD0013pTT5BamAvr


amp in E.coliCMV
pTT5

Mam
1B4
VD0014pTT5



CMV



Mam
1B5
VD0015pDAM02










1B6
VD0016pCAD04










1B7
VD0017pCAD09










1B8
VD0018MoClo#1

nStrep-TEVamp






1B9
VD0019MoClo#2

nStrep-MBP-TEVamp






1C1
VD0020pFastBac HT-AGibco12562-029n-term His6 + TEV siteamp in E.coliPolyhedrin



Insect
1C2
VD0021pFastBac HT-BGibco12562-029n-term His6 + TEV siteamp in E.coliPolyhedrin



Insect
1C3
VD0022pFastBac HT-CGibco12562-029n-term His6 + TEV siteamp in E.coliPolyhedrin



Insect
1C4
VD0023pFastBac HT-CATGibco12562-029CAT gene (chloramphenicol acetyltransferase)amp in E.coli



control pasmidInsect
1C5
VD0024pFastBac-DualGibco10712-024MCS - p10, Polyhedrin - MCSamp in E.coliPolyhedrin and p10


double expression vector with two promotorsInsect
1C6
VD0025pFastBac-Dual Gus/CATGibco10712-024chloramphenicol acetyltransferase and β-glucuronidase expression controlamp in E.coliPolyhedrin and p10


control plasmidInsect
1C7
VD0026pEG BacMamEric Gouaux/OHSU

amp in E.coliPolyhedrin, P10, CMV


Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1C8
VD0027pEG BacMam c-term 3c - eGFP - His8Eric Gouaux/OHSU
c-term 3c - eGFP - His8amp in E.coliPolyhedrin, P10, CMV


Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1C9
VD0028pEG BacMam n-term His8 - eGFP - 3cEric Gouaux/OHSU
n-term His8 - eGFP - 3camp in E.coliPolyhedrin, P10, CMV


Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1D1
VD0029pEZT-BMRyan Hibbs, Addgene74099Polyhedrin promoter replaced with CMV-containing cassette from pVLAD6 vector. Multiple cloning site replaced with custom. Synthetic, codon-optimized eGFP inserted after p10 promoteramp in E.coliP10, CMV


Multi-purpose rapid titer bacmam expression vector

1D2



  • No labels