The multi-purpose vector pFlpBtM contains elements necessary for the use as a donor vector for Flp-recombinase mediated cassette exchange (FRT = Flp recombinase target sites) and Tn7-transposition sequences for the generation of recombinant bacmids. FRTsites flank the region which is integrated into the RMCE locus in the master cell line. It contains the MCS and a downstream PGK-promoter for a selection trap method to screen for recombined cell clones. A larger section of the plasmid is integrated into the bacmids by TN7-based transposition. It contains the promoter region and a gentamicin-resistance gene for the selection of recombinant bacmids. The backbone of pFlpBtMII additionally contains an Epstein-Barr virus oriP for increased nuclear transport and episomal replication in EBNA positive cell lines. (doi:10.1371/journal.pone.0068674.g003)
- Snapgene file: VD0009_pFlpBtM II.dna
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