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Vector Map


  • For expression in mammalian cells

  • genes of interest are cloned into the multiple cloning site behind the CMV promoter using unique restriction sites

  • transcription initiation (CMV promoter)

  • transcription termination (SV40 poly-A late signal)

  • mRNA processing (intron and WPRE motif)

  • for insect cell expression and baculovirus amplification

  • including promoters (​polh and ​p10)

  • terminators (SV40 and HSV-​tk)

  • transposon elements (Tn7L and Tn7R)

  • resistance markers (​ampicillin and gentamicin)

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