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For expression in mammalian cells
genes of interest are cloned into the multiple cloning site behind the CMV promoter using unique restriction sites
transcription initiation (CMV promoter)
transcription termination (SV40 poly-A late signal)
mRNA processing (intron and WPRE motif)
for insect cell expression and baculovirus amplification
including promoters (polh and p10)
terminators (SV40 and HSV-tk)
transposon elements (Tn7L and Tn7R)
resistance markers (ampicillin and gentamicin)
- contains a n-term 8xHisTag followed by eGFP and 3c site
- made by Eric Gouaux/OHSU
- Snapgene file: VD0028_pEG BacMam n-term His8 - eGFP - 3c.dna
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