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Please find below a list of all vectors available in the PPCF for members of CSSB groups. Please note that due to the existence of restricting material transfer agreements (MTAs) for some vectors, those vectors can only be provided for use in the Protein Production Core Facility. If you are interested in using these vectors or strains in a lab other than the PPCF, please get in contact so we can help you to put all needed MTAs in place. Some MTAs are coupled with fees. We are happy to advise you on this issue.
NumberNameManufacture/OriginOrderNr.Architecture (Tag, Cleavage)DescriptionResistancePromotorOriTerminatorSignal SequenceBackboneseq. PrimerApplicationHostUsageBoxPositionMTA
VD0001pRSET His8


n-term His8, EK (Enterokinase)
ampT7




high-level prokaryotic expressionE.coli
1A1
VD0002pCoofy1Sabine Suppmann, Addgene43974n-term His6
kan



pETM14

E.coli
1A2
VD0003pCoofy18Sabine Suppmann, Addgene43975n-term His10
kan



pCoofy1

E.coli
1A3
VD0004pCoofy31Sabine Suppmann, Addgene43993n-term OneStrep, c-term His10
kan



pCoofy12



1A4
VD0005pCoofy61Sabine Suppmann, Addgene122013n-term His6
kan



pETM-NG



1A5
VD0006pFastBac1Joop van den Heuvel, HZI
Tn7L and Tn7R transposon sites
amp in E.coli, GentPolyhedrinf1, pUCSV40 polyA
pFastBac



1A6
VD0007pFastBacGSTJoop van den Heuvel, HZI
n-term GST, Tn7L and Tn7R transposon sites
amp in E.coli, GentPolyhedrinf1, pUCSV40 polyA
pFastBac



1A7
VD0008pcDNA3.1(+)Joop van den Heuvel, HZI


amp in E.coliCMVf1, pUCSV40 polyA


Mammalian expression vectorMam
1A8
VD0009pFlpBtm-IIJoop van den Heuvel, HZI
IgG-SP - MCS - TEV - OneStrep - His8
amp in E.coliCMVPMB1SV40 polyA





1A9
VD0010pET22b(+)BNITM, Sigma Aldrich69744-3N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HisTag
ampT7f1, pBR322
n-term pelB

pelB for potential periplasmic localizationE.coli
1B1
VD0011pET28c

His6 - Thrombin - MCS - His6
kanT7f1, pUC




E.coli
1B2
VD0012pETM44

His6 - MBP - 3c - MCS - His6
kanT7f1, pUC




E.coli
1B3
VD0013pTT5BamAvrJoop van den Heuvel, HZI


amp in E.coliCMV


pTT5

Mam
1B4
VD0014pTT5Joop van den Heuvel, HZI
TPL adenovirus tripartite leader

CMVPMB1beta globin poly (A) signal


expression in mammalian cellsMam
1B5
VD0015pDAM02













1B6
VD0016pCAD04













1B7
VD0017pCAD09













1B8
VD0018MoClo#1

nStrep-TEV
amp








1B9
VD0019MoClo#2

nStrep-MBP-TEV
amp








1C1
VD0020pFastBac HT-AGibco12562-029n-term His6 + TEV site
amp in E.coliPolyhedrin





Insect
1C2
VD0021pFastBac HT-BGibco12562-029n-term His6 + TEV site
amp in E.coliPolyhedrin





Insect
1C3
VD0022pFastBac HT-CGibco12562-029n-term His6 + TEV site
amp in E.coliPolyhedrin





Insect
1C4
VD0023pFastBac HT-CATGibco12562-029CAT gene (chloramphenicol acetyltransferase)
amp in E.coli





control pasmidInsect
1C5
VD0024pFastBac-DualGibco10712-024MCS - p10, Polyhedrin - MCS
amp in E.coliPolyhedrin and p10




double expression vector with two promotorsInsect
1C6
VD0025pFastBac-Dual Gus/CATGibco10712-024chloramphenicol acetyltransferase and β-glucuronidase expression control
amp in E.coliPolyhedrin and p10




control plasmidInsect
1C7
VD0026pEG BacMamEric Gouaux/OHSU


amp in E.coliPolyhedrin, P10, CMV




Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1C8
VD0027pEG BacMam c-term 3c - eGFP - His8Eric Gouaux/OHSU
c-term 3c - eGFP - His8
amp in E.coliPolyhedrin, P10, CMV




Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1C9
VD0028pEG BacMam n-term His8 - eGFP - 3cEric Gouaux/OHSU
n-term His8 - eGFP - 3c
amp in E.coliPolyhedrin, P10, CMV




Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1D1
VD0029pEZT-BMRyan Hibbs, Addgene74099Polyhedrin promoter replaced with CMV-containing cassette from pVLAD6 vector. Multiple cloning site replaced with custom. Synthetic, codon-optimized eGFP inserted after p10 promoter
amp in E.coliP10, CMV




Multi-purpose rapid titer bacmam expression vector

1D2Yes
VD0030pTTo3c-SEAPCNRC-NRC
SEAP (Secreted Embryonic Alkaline Phosphatase) reporter gene cloned into MCS, followed by c-term Strep-Tag II and His8Vector for monitoring transfection and productivity, SEAPamp in E.coliCMVpMB1, oriP



for HEK293-6E cellsMam
1D3
VD0031pYD11CNRC-NRC
Kozak Sequence, followed Signal Peptide (IgGk) - MCS - TEV Site - hFc (IgGk)Vector for transient expression with C-terminal His tag (for secretion), SP-MCS-TEV-Fcamp in E.coliCMVpMB1, oriP



for HEK293-6E cellsMam
1D4
VD0032pYD5CNRC-NRC
Kozak Sequence, followed by Signal Peptide (IgGk) - hFc (IgGk) - TEV - MCSVector for transient expression with N-terminal tag (for secretion), SP-Fc-TEV-MCSamp in E.coliCMVpMB1, oriP



for HEK293-6E cellsMam
1D5
VD0033pTT5-hIgG1CHCNRC-NRC
human IgG1 HC bewteen NheI and BamHIVector for VH and VL grafting on human IgG1 framework, GSoptamp in E.coliCMVpMB1, oriP



for HEK293-6E cellsMam
1D6
VD0034pTT5V5H8QCNRC-NRC
MCS followed by V5 and 8His (V5H8)Vector for transient expression with C-terminal tags, V5-His8Gamp in E.coliCMVpMB1



for HEK293-6E cellsMam
1D7
VD0035pTT5CNRC-NRC















VD0036pTT5SH8Q2CNRC-NRC
MCS followed by Strep-Tag II and 8HisVector for transient expression with C-terminal tags, StrepTagII-His8Gamp in E.coliCMVpMB1



for HEK293-6E cellsMam
1D9
VD0037pTT28CNRC-NRC
Kozak Sequence followed by MCS and 8HisVector for transient expression with C-terminal His tag (for secretion), SP-MCS-His8Gamp in E.coliCMVpMB1



for HEK293-6E cellsMam
1E1
VD0038pTT22SSP4CNRC-NRC
Kozak Seqence followed by 8His - TEV - NheI/BamHi cloning siteVector for transient expression with C-terminal His tag (for secretion), SP-His8G-TEV-MCSamp in E.coliCMVpMB1



for HEK293-6E cellsMam
1E2
VD0039pTT5-hVLCLkCNRC-NRC
hVLCKappa (GSopt) cloned between EcoRI/BamHIVector for VH and VL grafting on human IgG1 framework, GSoptamp in E.coliCMVpMB1, oriP



for HEK293-6E cellsMam
1E3
VD0040pTTo-GFPCNRC-NRC

Vector for monitoring transfection and productivity, GFPamp in E.coliCMVpMB1, oriP



for HEK293-6E cellsMam
1E4
VD0041pTTVH8GCNRC-NRC

Vector for transient expression with N-terminal tag (for secretion), SP-His8G-MCSamp in E.coliCMVpBM1



for HEK293-6E cellsMam
1E5
VD0042pFastBac1-MVKYS-Strep2-TEV
MVKYS enhacer sequence followed by Strep2 Tag and TEV site - MCS
amp in E.coliPolyhedrin





Insect
1E6
VD0043pFastBac Dual-MVKYS-Strep2-TEVPhilipp Lewe
polyhedrin promotor followed by MVKYS enhacer sequence followed by Strep2 Tag and TEV site - MCS
amp in E.coliPolyhedrin, P10





Insect
1E7
VD0044pSecTag2CInvitrogenV90020
IgK Secretion Sequence followed by MCS - c-term c-MYC - c-term 6x HisTag
amp in E.coliCMV
SV40


expression in mammalian cells, secreting fusion protein into medium with IgK secretion sequenceMam
1E8
VD0045pET22b
















VD0046pCMV2.5-hIgG1-Fc-XPMichael Hust, TU-BS


amp in E.coliCMVpUC




Mam
1E9
VD0047pLeGo-IG2Addgene, Svenja Schneegans27341

amp in E.coli








1F1