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Please find below a list of all vectors available in the PPCF for members of CSSB groups. Please note that due to the existence of restricting material transfer agreements (MTAs) for some vectors, those vectors can only be provided for use in the Protein Production Core Facility. If you are interested in using these vectors or strains in a lab other than the PPCF, please get in contact so we can help you to put all needed MTAs in place. Some MTAs are coupled with fees. We are happy to advise you on this issue.
NumberNameManufacture/OriginOrderNr.Architecture (Tag, Cleavage)ResistancePromotorOriTerminatorSignal SequenceBackboneseq. PrimerApplicationHostUsageBoxPosition
VD0001pRSET His8


n-term His8, EK (Enterokinase)ampT7




high-level prokaryotic expressionE.coli
1A1
VD0002pCoofy1Sabine Suppmann, Addgene43974n-term His6kan



pETM14

E.coli
1A2
VD0003pCoofy18Sabine Suppmann, Addgene43975n-term His10kan



pCoofy1

E.coli
1A3
VD0004pCoofy31Sabine Suppmann, Addgene43993n-term OneStrep, c-term His10kan



pCoofy12



1A4
VD0005pCoofy61Sabine Suppmann, Addgene122013n-term His6kan



pETM-NG



1A5
VD0006pFastBac1Joop van den Heuvel, HZI
Tn7L and Tn7R transposon sitesamp in E.coli, GentPolyhedrinf1, pUCSV40 polyA
pFastBac



1A6
VD0007pFastBacGSTJoop van den Heuvel, HZI
n-term GST, Tn7L and Tn7R transposon sitesamp in E.coli, GentPolyhedrinf1, pUCSV40 polyA
pFastBac



1A7
VD0008pcDNA3.1(+)Joop van den Heuvel, HZI

amp in E.coliCMVf1, pUCSV40 polyA


Mammalian expression vectorMam
1A8
VD0009pFlpBtm-IIJoop van den Heuvel, HZI
IgG-SP - MCS - TEV - OneStrep - His8amp in E.coliCMVPMB1SV40 polyA





1A9
VD0010pET22b(+)BNITM, Sigma Aldrich69744-3c-term His6ampT7f1, pBR322
n-term pelB

pelB for potential periplasmic localizationE.coli
1B1
VD0011pET28c

His6 - Thrombin - MCS - His6kanT7f1, pUC




E.coli
1B2
VD0012pETM44

His6 - MBP - 3c - MCS - His6kanT7f1, pUC




E.coli
1B3
VD0013pTT5BamAvrJoop van den Heuvel, HZI

amp in E.coliCMV


pTT5

Mam
1B4
VD0014pTT5Joop van den Heuvel, HZI
TPL adenovirus tripartite leader
CMVPMB1beta globin poly (A) signal


expression in mammalian cellsMam
1B5
VD0015pDAM02












1B6
VD0016pCAD04












1B7
VD0017pCAD09












1B8
VD0018MoClo#1

nStrep-TEVamp








1B9
VD0019MoClo#2

nStrep-MBP-TEVamp








1C1
VD0020pFastBac HT-AGibco12562-029n-term His6 + TEV siteamp in E.coliPolyhedrin





Insect
1C2
VD0021pFastBac HT-BGibco12562-029n-term His6 + TEV siteamp in E.coliPolyhedrin





Insect
1C3
VD0022pFastBac HT-CGibco12562-029n-term His6 + TEV siteamp in E.coliPolyhedrin





Insect
1C4
VD0023pFastBac HT-CATGibco12562-029CAT gene (chloramphenicol acetyltransferase)amp in E.coli





control pasmidInsect
1C5
VD0024pFastBac-DualGibco10712-024MCS - p10, Polyhedrin - MCSamp in E.coliPolyhedrin and p10




double expression vector with two promotorsInsect
1C6
VD0025pFastBac-Dual Gus/CATGibco10712-024chloramphenicol acetyltransferase and β-glucuronidase expression controlamp in E.coliPolyhedrin and p10




control plasmidInsect
1C7
VD0026pEG BacMamEric Gouaux/OHSU

amp in E.coliPolyhedrin, P10, CMV




Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1C8
VD0027pEG BacMam c-term 3c - eGFP - His8Eric Gouaux/OHSU
c-term 3c - eGFP - His8amp in E.coliPolyhedrin, P10, CMV




Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1C9
VD0028pEG BacMam n-term His8 - eGFP - 3cEric Gouaux/OHSU
n-term His8 - eGFP - 3camp in E.coliPolyhedrin, P10, CMV




Virus formation in Insect cells to infect Mammalian cellsInsect, Mam
1D1
VD0029pEZT-BMRyan Hibbs, Addgene74099Polyhedrin promoter replaced with CMV-containing cassette from pVLAD6 vector. Multiple cloning site replaced with custom. Synthetic, codon-optimized eGFP inserted after p10 promoteramp in E.coliP10, CMV




Multi-purpose rapid titer bacmam expression vector

1D2