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Please find below a list of all vectors available in the PPCF for members of CSSB groups. Please note that due to the existence of restricting material transfer agreements (MTAs) for some vectors, those vectors can only be provided for use in the Protein Production Core Facility. If you are interested in using these vectors or strains in a lab other than the PPCF, please get in contact so we can help you to put all needed MTAs in place. Some MTAs are coupled with fees. We are happy to advise you on this issue.
NumberNameManufacture/OriginOrderNr.Architecturen-term Affinityc-term AffinityDescriptionResistancePromotorOriTerminatorSignal SequenceBackboneApplicationHostBoxPositionMTA
VD0001pRSET His8


n-term His8, EK (Enterokinase)8x His

ampT7



high-level prokaryotic expressionE.coli1A1
VD0002pCoofy1Sabine Suppmann, Addgene439746x His - 3c site - MCS - ccdb - 6x His6x His6x His
kan



pETM14
E.coli1A2
VD0003pCoofy18Sabine Suppmann, Addgene4397510x His - 3c site - MCS - ccdb - 6x His10x His6x His
kan



pCoofy1
E.coli1A3
VD0004pCoofy31Sabine Suppmann, Addgene43993TwinStrepII - 3c site - MCS - ccdb - 6x HisTwinStrepII10x His
kan



pCoofy12

1A4
VD0005pCoofy61Sabine Suppmann, Addgene1220136x His - eGFP - 3c site - MCS - ccdb - 6x His6x His6x His
kan



pETM-NG

1A5
VD0006pFastBac1Joop van den Heuvel, HZI
Tn7L and Tn7R transposon sites


amp in E.coli, GentPolyhedrinf1, pUCSV40 polyA
pFastBac

1A6
VD0007pFastBacGSTJoop van den Heuvel, HZI
n-term GST, Tn7L and Tn7R transposon sitesGST

amp in E.coli, GentPolyhedrinf1, pUCSV40 polyA
pFastBac

1A7
VD0008pcDNA3.1(-)Joop van den Heuvel, HZI
MCS


amp in E.coliCMVf1, pUCSV40 polyA

Mammalian expression vectorMam1A8
VD0009pFlpBtm-IIJoop van den Heuvel, HZI
IgG-SP - MCS - TEV site - OneStrep - 8x His
8x HisIgG signal peptide before MCSamp in E.coliCMVPMB1SV40 polyA



1A9
VD0010pET22b(+)BNITM, Sigma Aldrich69744-3pelB SP - MCS - 6x His
6x HispelB signal peptide for periplasmic expressionampT7f1, pBR322
n-term pelB
pelB for potential periplasmic localizationE.coli1B1
VD0011pET28c

6x His - Thrombin site - T7 Tag - MCS - 6x His6x His6x His
kanT7f1, pUC



E.coli1B2
VD0012pETM44

6x His - MBP - 3c site - MCS - 6x His6x His6x His
kanT7f1, pUC



E.coli1B3
VD0013pTT5BamAvrJoop van den Heuvel, HZI
TPL leader - MCS


amp in E.coliCMV


pTT5
Mam1B4
VD0014pTT5Joop van den Heuvel, HZI
TPL leader - MCS



CMVPMB1beta globin poly (A) signal

expression in mammalian cellsMam1B5
VD0015pDAM02













1B6
VD0016pCAD04













1B7
VD0017pCAD09













1B8
VD0018MoClo#1

StrepII - TEV siteStrepII

amp






1B9
VD0019MoClo#2

StrepII - MBP - Tev siteStrepII

amp






1C1
VD0020pFastBac HT-AGibco12562-0296x His - TEV site - MCS6x His

amp in E.coliPolyhedrin




Insect1C2
VD0021pFastBac HT-BGibco12562-0296x His - TEV site - MCS6x His

amp in E.coliPolyhedrin




Insect1C3
VD0022pFastBac HT-CGibco12562-0296x His - TEV site - MCS6x His

amp in E.coliPolyhedrin




Insect1C4
VD0023pFastBac HT-CATGibco12562-029CAT gene (chloramphenicol acetyltransferase)


amp in E.coli




control pasmidInsect1C5
VD0024pFastBac-DualGibco10712-024MCS - p10, Polyhedrin - MCS


amp in E.coliPolyhedrin and p10



double expression vector with two promotorsInsect1C6
VD0025pFastBac-Dual Gus/CATGibco10712-024chloramphenicol acetyltransferase and β-glucuronidase expression control


amp in E.coliPolyhedrin and p10



control plasmidInsect1C7
VD0026pEG BacMamEric Gouaux/OHSU
MCS - p10, Polyhedrin - CMV - MCS


amp in E.coliPolyhedrin, P10, CMV



Virus formation in Insect cells to infect Mammalian cellsInsect, Mam1C8
VD0027pEG BacMam c-term 3c - eGFP - His8Eric Gouaux/OHSU
MCS - p10, Polyhedrin - CMV - MCS - 3c site - GFP - 8x His
8x His
amp in E.coliPolyhedrin, P10, CMV



Virus formation in Insect cells to infect Mammalian cellsInsect, Mam1C9
VD0028pEG BacMam n-term His8 - eGFP - 3cEric Gouaux/OHSU
MCS - p10, Polyhedrin - CMV - 8x His - MCS - 3c site - GFP8x His

amp in E.coliPolyhedrin, P10, CMV



Virus formation in Insect cells to infect Mammalian cellsInsect, Mam1D1
VD0029pEZT-BMRyan Hibbs, Addgene74099p10 - meGFP, CMV - MCS

Polyhedrin promoter replaced with CMV-containing cassette from pVLAD6 vector. Multiple cloning site replaced with custom. Synthetic, codon-optimized eGFP inserted after p10 promoteramp in E.coliP10, CMV



Multi-purpose rapid titer bacmam expression vector
1D2Yes
VD0030pTTo3c-SEAPCNRC-NRC
CMV - SEAP SH8Q1 - StrepII - 8x His
StrepII, 8x HisSEAP (Secreted Embryonic Alkaline Phosphatase) reporter gene cloned into MCS, followed by c-term Strep-Tag II and His8, Vector for monitoring transfection and productivity, SEAPamp in E.coliCMVpMB1, oriP


for HEK293-6E cellsMam1D3
VD0031pYD11CNRC-NRC
IgGk SP - MCS - TEV site - hFc (IgGk)

Vector for transient expression with C-terminal His tag (for secretion), SP-MCS-TEV-Fcamp in E.coliCMVpMB1, oriP


for HEK293-6E cellsMam1D4
VD0032pYD5CNRC-NRC
IgGk SP - hFc (IgGk) - TEV site - MCS

Vector for transient expression with N-terminal tag (for secretion), SP-Fc-TEV-MCSamp in E.coliCMVpMB1, oriP


for HEK293-6E cellsMam1D5
VD0033pTT5-hIgG1CHCNRC-NRC
CMV - hIgG 1CH (GSopt)

Vector for VH and VL grafting on human IgG1 framework, GSopt, human IgG1 HC bewteen NheI and BamHI
amp in E.coliCMVpMB1, oriP


for HEK293-6E cellsMam1D6
VD0034pTT5V5H8QCNRC-NRC
CMV - MCS - V5 Tag - 8x His
V5, 8x HisVector for transient expression with C-terminal tags, V5-His8Gamp in E.coliCMVpMB1


for HEK293-6E cellsMam1D7
VD0035pTT5CNRC-NRC















VD0036pTT5SH8Q2CNRC-NRC
CMV - MCS - StrepII - 8x His
StrepII, 8x HisVector for transient expression with C-terminal tags, StrepTagII-His8Gamp in E.coliCMVpMB1


for HEK293-6E cellsMam1D9
VD0037pTT28CNRC-NRC
MCS - 8x His
8x HisVector for transient expression with C-terminal His tag (for secretion), SP-MCS-His8Gamp in E.coliCMVpMB1


for HEK293-6E cellsMam1E1
VD0038pTT22SSP4CNRC-NRC
8x His - TEV site - MCS (NheI+BamHI)8x His
Vector for transient expression with C-terminal His tag (for secretion), SP-His8G-TEV-MCSamp in E.coliCMVpMB1


for HEK293-6E cellsMam1E2
VD0039pTT5-hVLCLkCNRC-NRC
CMV - hVLC Kappa

Vector for VH and VL grafting on human IgG1 framework, GSopt, hVLCKappa (GSopt) cloned between EcoRI/BamHI
amp in E.coliCMVpMB1, oriP


for HEK293-6E cellsMam1E3
VD0040pTTo-GFPCNRC-NRC
GFP in MCS

Vector for monitoring transfection and productivity, GFPamp in E.coliCMVpMB1, oriP


for HEK293-6E cellsMam1E4
VD0041pTTVH8GCNRC-NRC
VEGF SP - 8x His - MCS8x His
Vector for transient expression with N-terminal tag (for secretion)amp in E.coliCMVpBM1


for HEK293-6E cellsMam1E5
VD0042pFastBac1-MVKYS-Strep2-TEV
MVKYS - StrepII - TEV site - MCSStrepII

amp in E.coliPolyhedrin




Insect1E6
VD0043pFastBac Dual-MVKYS-Strep2-TEVPhilipp Lewe
MCS - p10, Polyhedrin - MVKYS - StrepII - MCSStrepII

amp in E.coliPolyhedrin, P10




Insect1E7
VD0044pSecTag2CInvitrogenV90020
IgK Leader - MCS - MYC - 6x His
6x HisIgK secretion leader before MCSamp in E.coliCMV
SV40

expression in mammalian cells, secreting fusion protein into medium with IgK secretion sequenceMam1E8
VD0045pET22b
















VD0046pCMV2.5-hIgG1-Fc-XPMichael Hust, TU-BS
IgG1 FC bewteen NheI+XbaI


amp in E.coliCMVpUC



Mam1E9
VD0047pLeGo-IG2Addgene27341



amp in E.coli






1F1
VD0048pET21a
















VD0049pMaL-c6TNEB