Please find below a list of all vectors available in the PPCF for members of CSSB groups. Please note that due to the existence of restricting material transfer agreements (MTAs) for some vectors, those vectors can only be provided for use in the Protein Production Core Facility. If you are interested in using these vectors or strains in a lab other than the PPCF, please get in contact so we can help you to put all needed MTAs in place. Some MTAs are coupled with fees. We are happy to advise you on this issue.
| Number | Name | Manufacture/Origin | OrderNr. | Architecture (Tag, Cleavage) | Description | Resistance | Promotor | Ori | Terminator | Signal Sequence | Backbone | seq. Primer | Application | Host | Usage | Box | Position | MTA |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VD0001 | pRSET His8 | n-term His8, EK (Enterokinase) | amp | T7 | high-level prokaryotic expression | E.coli | 1 | A1 | ||||||||||
| VD0002 | pCoofy1 | Sabine Suppmann, Addgene | 43974 | n-term His6 | kan | pETM14 | E.coli | 1 | A2 | |||||||||
| VD0003 | pCoofy18 | Sabine Suppmann, Addgene | 43975 | n-term His10 | kan | pCoofy1 | E.coli | 1 | A3 | |||||||||
| VD0004 | pCoofy31 | Sabine Suppmann, Addgene | 43993 | n-term OneStrep, c-term His10 | kan | pCoofy12 | 1 | A4 | ||||||||||
| VD0005 | pCoofy61 | Sabine Suppmann, Addgene | 122013 | n-term His6 | kan | pETM-NG | 1 | A5 | ||||||||||
| VD0006 | pFastBac1 | Joop van den Heuvel, HZI | Tn7L and Tn7R transposon sites | amp in E.coli, Gent | Polyhedrin | f1, pUC | SV40 polyA | pFastBac | 1 | A6 | ||||||||
| VD0007 | pFastBacGST | Joop van den Heuvel, HZI | n-term GST, Tn7L and Tn7R transposon sites | amp in E.coli, Gent | Polyhedrin | f1, pUC | SV40 polyA | pFastBac | 1 | A7 | ||||||||
| VD0008 | pcDNA3.1(+) | Joop van den Heuvel, HZI | amp in E.coli | CMV | f1, pUC | SV40 polyA | Mammalian expression vector | Mam | 1 | A8 | ||||||||
| VD0009 | pFlpBtm-II | Joop van den Heuvel, HZI | IgG-SP - MCS - TEV - OneStrep - His8 | amp in E.coli | CMV | PMB1 | SV40 polyA | 1 | A9 | |||||||||
| VD0010 | pET22b(+) | BNITM, Sigma Aldrich | 69744-3 | N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HisTag | amp | T7 | f1, pBR322 | n-term pelB | pelB for potential periplasmic localization | E.coli | 1 | B1 | ||||||
| VD0011 | pET28c | His6 - Thrombin - MCS - His6 | kan | T7 | f1, pUC | E.coli | 1 | B2 | ||||||||||
| VD0012 | pETM44 | His6 - MBP - 3c - MCS - His6 | kan | T7 | f1, pUC | E.coli | 1 | B3 | ||||||||||
| VD0013 | pTT5BamAvr | Joop van den Heuvel, HZI | amp in E.coli | CMV | pTT5 | Mam | 1 | B4 | ||||||||||
| VD0014 | pTT5 | Joop van den Heuvel, HZI | TPL adenovirus tripartite leader | CMV | PMB1 | beta globin poly (A) signal | expression in mammalian cells | Mam | 1 | B5 | ||||||||
| VD0015 | pDAM02 | 1 | B6 | |||||||||||||||
| VD0016 | pCAD04 | 1 | B7 | |||||||||||||||
| VD0017 | pCAD09 | 1 | B8 | |||||||||||||||
| VD0018 | MoClo#1 | nStrep-TEV | amp | 1 | B9 | |||||||||||||
| VD0019 | MoClo#2 | nStrep-MBP-TEV | amp | 1 | C1 | |||||||||||||
| VD0020 | pFastBac HT-A | Gibco | 12562-029 | n-term His6 + TEV site | amp in E.coli | Polyhedrin | Insect | 1 | C2 | |||||||||
| VD0021 | pFastBac HT-B | Gibco | 12562-029 | n-term His6 + TEV site | amp in E.coli | Polyhedrin | Insect | 1 | C3 | |||||||||
| VD0022 | pFastBac HT-C | Gibco | 12562-029 | n-term His6 + TEV site | amp in E.coli | Polyhedrin | Insect | 1 | C4 | |||||||||
| VD0023 | pFastBac HT-CAT | Gibco | 12562-029 | CAT gene (chloramphenicol acetyltransferase) | amp in E.coli | control pasmid | Insect | 1 | C5 | |||||||||
| VD0024 | pFastBac-Dual | Gibco | 10712-024 | MCS - p10, Polyhedrin - MCS | amp in E.coli | Polyhedrin and p10 | double expression vector with two promotors | Insect | 1 | C6 | ||||||||
| VD0025 | pFastBac-Dual Gus/CAT | Gibco | 10712-024 | chloramphenicol acetyltransferase and β-glucuronidase expression control | amp in E.coli | Polyhedrin and p10 | control plasmid | Insect | 1 | C7 | ||||||||
| VD0026 | pEG BacMam | Eric Gouaux/OHSU | amp in E.coli | Polyhedrin, P10, CMV | Virus formation in Insect cells to infect Mammalian cells | Insect, Mam | 1 | C8 | ||||||||||
| VD0027 | pEG BacMam c-term 3c - eGFP - His8 | Eric Gouaux/OHSU | c-term 3c - eGFP - His8 | amp in E.coli | Polyhedrin, P10, CMV | Virus formation in Insect cells to infect Mammalian cells | Insect, Mam | 1 | C9 | |||||||||
| VD0028 | pEG BacMam n-term His8 - eGFP - 3c | Eric Gouaux/OHSU | n-term His8 - eGFP - 3c | amp in E.coli | Polyhedrin, P10, CMV | Virus formation in Insect cells to infect Mammalian cells | Insect, Mam | 1 | D1 | |||||||||
| VD0029 | pEZT-BM | Ryan Hibbs, Addgene | 74099 | Polyhedrin promoter replaced with CMV-containing cassette from pVLAD6 vector. Multiple cloning site replaced with custom. Synthetic, codon-optimized eGFP inserted after p10 promoter | amp in E.coli | P10, CMV | Multi-purpose rapid titer bacmam expression vector | 1 | D2 | Yes | ||||||||
| VD0030 | pTTo3c-SEAP | CNRC-NRC | SEAP (Secreted Embryonic Alkaline Phosphatase) reporter gene cloned into MCS, followed by c-term Strep-Tag II and His8 | Vector for monitoring transfection and productivity, SEAP | amp in E.coli | CMV | pMB1, oriP | for HEK293-6E cells | Mam | 1 | D3 | |||||||
| VD0031 | pYD11 | CNRC-NRC | Kozak Sequence, followed Signal Peptide (IgGk) - MCS - TEV Site - hFc (IgGk) | Vector for transient expression with C-terminal His tag (for secretion), SP-MCS-TEV-Fc | amp in E.coli | CMV | pMB1, oriP | for HEK293-6E cells | Mam | 1 | D4 | |||||||
| VD0032 | pYD5 | CNRC-NRC | Kozak Sequence, followed by Signal Peptide (IgGk) - hFc (IgGk) - TEV - MCS | Vector for transient expression with N-terminal tag (for secretion), SP-Fc-TEV-MCS | amp in E.coli | CMV | pMB1, oriP | for HEK293-6E cells | Mam | 1 | D5 | |||||||
| VD0033 | pTT5-hIgG1CH | CNRC-NRC | human IgG1 HC bewteen NheI and BamHI | Vector for VH and VL grafting on human IgG1 framework, GSopt | amp in E.coli | CMV | pMB1, oriP | for HEK293-6E cells | Mam | 1 | D6 | |||||||
| VD0034 | pTT5V5H8Q | CNRC-NRC | MCS followed by V5 and 8His (V5H8) | Vector for transient expression with C-terminal tags, V5-His8G | amp in E.coli | CMV | pMB1 | for HEK293-6E cells | Mam | 1 | D7 | |||||||
| VD0035 | pTT5 | CNRC-NRC | ||||||||||||||||
| VD0036 | pTT5SH8Q2 | CNRC-NRC | MCS followed by Strep-Tag II and 8His | Vector for transient expression with C-terminal tags, StrepTagII-His8G | amp in E.coli | CMV | pMB1 | for HEK293-6E cells | Mam | 1 | D9 | |||||||
| VD0037 | pTT28 | CNRC-NRC | Kozak Sequence followed by MCS and 8His | Vector for transient expression with C-terminal His tag (for secretion), SP-MCS-His8G | amp in E.coli | CMV | pMB1 | for HEK293-6E cells | Mam | 1 | E1 | |||||||
| VD0038 | pTT22SSP4 | CNRC-NRC | Kozak Seqence followed by 8His - TEV - NheI/BamHi cloning site | Vector for transient expression with C-terminal His tag (for secretion), SP-His8G-TEV-MCS | amp in E.coli | CMV | pMB1 | for HEK293-6E cells | Mam | 1 | E2 | |||||||
| VD0039 | pTT5-hVLCLk | CNRC-NRC | hVLCKappa (GSopt) cloned between EcoRI/BamHI | Vector for VH and VL grafting on human IgG1 framework, GSopt | amp in E.coli | CMV | pMB1, oriP | for HEK293-6E cells | Mam | 1 | E3 | |||||||
| VD0040 | pTTo-GFP | CNRC-NRC | Vector for monitoring transfection and productivity, GFP | amp in E.coli | CMV | pMB1, oriP | for HEK293-6E cells | Mam | 1 | E4 | ||||||||
| VD0041 | pTTVH8G | CNRC-NRC | Vector for transient expression with N-terminal tag (for secretion), SP-His8G-MCS | amp in E.coli | CMV | pBM1 | for HEK293-6E cells | Mam | 1 | E5 | ||||||||
| VD0042 | pFastBac1-MVKYS-Strep2-TEV | MVKYS enhacer sequence followed by Strep2 Tag and TEV site - MCS | amp in E.coli | Polyhedrin | Insect | 1 | E6 | |||||||||||
| VD0043 | pFastBac Dual-MVKYS-Strep2-TEV | Philipp Lewe | polyhedrin promotor followed by MVKYS enhacer sequence followed by Strep2 Tag and TEV site - MCS | amp in E.coli | Polyhedrin, P10 | Insect | 1 | E7 | ||||||||||
| VD0044 | pSecTag2C | Invitrogen | V90020 | IgK Secretion Sequence followed by MCS - c-term c-MYC - c-term 6x HisTag | amp in E.coli | CMV | SV40 | expression in mammalian cells, secreting fusion protein into medium with IgK secretion sequence | Mam | 1 | E8 | |||||||
| VD0045 | pET22b | |||||||||||||||||
| VD0046 | pCMV2.5-hIgG1-Fc-XP | Michael Hust, TU-BS | amp in E.coli | CMV | pUC | Mam | 1 | E9 | ||||||||||
| VD0047 | pLeGo-IG2 | Addgene, Svenja Schneegans | 27341 | amp in E.coli | 1 | F1 |
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